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SOLVED: The autoradiogram shown was obtained from a DNA sequencing method called Sanger sequencing or dideoxy sequencing. The arrow shows the direction of migration of the DNA samples during electrophoresis.ddATPddCTPddGTPddTTPDetermine the sequence of the DNA template strand, in the 5' to 3' direction, from this data.5' - ctaaacttgcgIncorrect
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Sanger Sequencing Logic In the Sanger (dideoxy) method for DNA sequencing, a small amount of a dideoxynucleoside triphosphate-say, ddCTP-is added to the sequencing reaction along with a larger amount of the corresponding dCTP. What result would be observed if the dCTP were omitted? | Numerade
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